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ChBE Seminar: The Role of Intrinsically Disordered Proteins in Sensing Membrane Curvature Speaker: Wade Zeno, Assistant Professor, Mork Family Dept of Chemical Engineering & Materials Science, University of Southern California Abstract: The ability of proteins to sense membrane curvature is essential for the initiation and assembly of curved membrane structures in a variety of cellular processes. Established mechanisms of curvature sensing rely on proteins with specific structural features such as amphipathic helices. In contrast, we have recently discovered that intrinsically disordered proteins (IDPs), which lack a defined three-dimensional fold, can also be potent sensors of membrane curvature. This ability of IDPs to sense curvature arises from two key physical features – a high degree of conformational entropy and a high net negative charge. Binding of such IDPs to membrane surfaces results simultaneously in a decrease in conformational entropy and an increase in electrostatic repulsion by anionic lipids. Here we show that each of these effects gives rise to a distinct mechanism of curvature sensing. Specifically, as the curvature of the membrane increases, the steric constraint that it imposes on the conformation of the IDP is reduced, leading to an entropic preference for curved membranes. At the same time, increasing membrane curvature increases the average separation between anionic amino acids and anionic lipids, leading to an electrostatic preference for curved membranes. To examine curvature sensitivity by IDPs, we engineered various truncation and chimeric mutants that were derived from the endocytic proteins AP180, Epsin1, and Amphiphysin1. Using Monte Carlo simulation and quantitative in vitro fluorescence techniques, our results demonstrate that long IDP chains with relatively low net charge sense membrane curvature predominately through the entropic mechanism, while shorter, more highly charged IDP chains rely largely on the electrostatic mechanism. We also demonstrate that IDPs can sense membrane curvature in live cells. Finally, we show that full-length endocytic proteins, which contain both structured curvature sensors and disordered regions, are more than twice as curvature sensitive as their respective structured domains alone. These findings demonstrate curvature sensing mechanisms that are independent of protein structure and illustrate how structured and disordered domains can collaborate to synergistically enhance curvature sensitivity. Biography: Wade Zeno joined the Mork Family Department of Chemical Engineering and Materials Science at the University of Southern California in Fall 2020 as an assistant professor. He earned his PhD in Chemical Engineering from the University of California, Davis in 2016 and worked as a postdoctoral fellow in the Biomedical Engineering Department at the University of Texas at Austin until 2020. His research expertise is in biological membrane engineering. Specifically, he examines the molecules that comprise cellular membranes (i.e. proteins and lipids) to understand (i) how they function at a fundamental level and (ii) how they can be exploited to make functional biomaterials. The broader impacts of this work are far-reaching, ranging from understanding viral infection and disease to developing and delivering therapeutics. Twitter Handle: @ZenoResearch This Event is For: Campus
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